This advisor describes the consequences of various variables on shrinkage and creep of hardened concrete, together with mix proportions, atmosphere, layout, and development. This record is geared toward designers who desire to achieve extra information regarding elements altering shrinkage and creep yet doesn't comprise info at the prediction of shrinkage and creep or structural layout concerns linked to shrinkage and creep.
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Extra resources for ACI 209.1R-05: Report on Factors Affecting Shrinkage and Creep of Hardened Concrete
The boxes should be disinfected and then scrubbed clean at regular intervals. Chapter 5 Examination of direct smears Direct microscopical examination of pathological material for acid-fast bacilli may be a valuable or quite useless procedure depending on the nature and quality of the specimen. If this is satisfactory it is either spread directly on a microscope slide or concentrated by centrifugation, if necessary after treatment to lower its specific gravity so that any mycobacteria are more easily sedimented.
Violent action may disturb the arrangement of the bacilli (see below). At this point the ease with which the organisms emulsify in the liquid should be noted. Tubercle bacilli, for example, do not form smooth suspensions, unlike some other mycobacteria. The films are allowed to dry and are then fixed by heat and stained by the ZN method. ) are being stained. 1. shows the microscopical appearance of several species of mycobacteria. The arrangement of the cells varies from the 'serpentine cords' usually, but not always, characteristic of tubercle bacilli, to a uniform distribution of cells.
Certain contaminating organisms produce acid from constituents of the medium and the lowering of the pH unbinds some of the malachite green from the egg. The colour of the medium changes to dark green. Tubercle bacilli will not grow under these conditions. Slopes showing confluent growth or isolated colonies of a buff, yellow or orange colour should be removed for microscopical examination. Close inspection, preferably with a hand lens and under a good bench lamp, is necessary to reveal very small colonies or the effuse growth which is characteristic of some opportunist mycobacteria.