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By Michael Krings, Giuseppe Montana, Richard Dronskowski, Claudia Wickleder

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12 (1991) Copyright © 1991 AOCS Press 988 G. GORACCI AND E. FRANCESCANGELI FIG. 2. Effect of microsomal protein concentration on the synthesis of PAF and dioctanoyl-GPC. 1 mM labeled CDPcholine (spec. act. 3 mL. PAF-synthesizing phosphocholine-transferase was assayed in the presence of 20 mM DTT. Data are from one experiment in duplicate. FIG. 3. Dependence of brain phosphocholinetransferase activity on CDPcholine concentration. 005% Tween 20, 20 mM MgCl2, labeled CDPcholine (spec. act. 3 mL. PAF-synthesizing phosphocholinetransferase was assayed in the presence of 20 mM DTT.

Data represent the mean of duplicate determinations from one experiment which is representative of another which gave similar results. 15 were resuspended in 90 mM Tris/HCl (pH 8), or in the same buffer containing 20 mM DTT, and preincubated at 60°C for the indicated periods of time. 1 mM labeled CDPcholine (spec. act. 66 nCi/nmol) and 70 μg microsomal protein. Data (mean of duplicate samples) are expressed as % of the enzyme specific activity of non preincubated samples. DO-GPC, dioctanoyl-GPC.

Res. Commun. 120, 834–839. 24. , and Mandel, P. (1980) J. Neurochem. 34, 963–969. 25. A. (1977) J. Lipid Res. 18, 53–58. 26. C. (1981) Biochem. J. 195, 301–306. 27. , and Snyder, F. (1983) J. Biol. Chem. 258, 97–102. 28. , and Porcellati, G. (1974) HoppeSeyler’s Z. Physiol. Chem. 355, 75–81. 29. , and Porcellati, G. (1974) Hoppe-Seyler’s Z. Physiol. Chem. 355, 1535–1542. 30. , and Porcellati G. (1973) Hoppe-Seyler’s Z. Physiol. Chem. 354, 729–736. 31. , and Arienti, G. (1970) Lipids 5, 725–733.

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